Handle Products with Caution
HPLC vs. GC-MS: Understanding Purity Verification Methods
HPLC vs. GC-MS: Understanding Purity Verification Methods
How analytical chemistry ensures the ≥98% purity standard of ELITE CHEMS research compounds.
Scientific review by DR. James, Analytical Chemistry Director
When you receive a Certificate of Analysis (CoA) with your order, it represents hours of advanced instrumentation work. Here's how we validate purity.
HPLC: The Gold Standard
▲ How It Works
High-Performance Liquid Chromatography separates compounds using:
- Polarity gradients in mobile phase
- C18 reverse-phase columns
- UV-VIS detection (190-400nm)
◉ Our Protocol
All batch-tested products undergo:
- Triplicate injections
- USP-grade reference standards
- 0.5% RSD threshold
GC-MS: Volatile Compound Analysis
When We Use GC-MS
For low-molecular-weight compounds like:
- Cathinones (MW < 250)
- Piperazines
- Volatile novel analogs
Detection limits: 0.1% impurity threshold with EI ionization
Side-by-Side Comparison
| Parameter | HPLC-UV | GC-MS |
|---|---|---|
| Ideal for | Peptides, polar compounds | Volatile small molecules |
| Detection Limit | 0.5% impurities | 0.1% impurities |
| Sample Prep | Dissolution in mobile phase | Derivatization often needed |
Request Your CoA
Every ELITE CHEMS product includes batch-specific purity data. Contact us for full analytical reports.
VIEW CERTIFIED PRODUCTSWhy This Matters for Your Research
▼ Reproducible Results
Our HPLC-tested peptides eliminate batch variability
▼ Impurity Profiling
GC-MS identifies synthesis byproducts in synthetic cannabinoids
Technical Deep Dive
Our lab uses these instrument configurations:
HPLC Specifications
- System: Agilent 1260 Infinity II
- Column: Zorbax Eclipse Plus C18 (4.6×150mm, 3.5µm)
- Method: 10-90% ACN in H2O (0.1% TFA) over 20min
For researchers requiring analytical documentation, we provide:
- Raw chromatograms upon request
- Method validation reports for bulk orders
